Gamma-sitosterol from Ficus Exasperata ameliorated the pathophysiological complications of type 1 diabetes mellitus in albino rats
Adeyi Akindele Oluwatosin1,&, Gloria Izu1
1Animal Physiology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria
Adeyi Akindele Oluwatosin, Animal Physiology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria
our previous studies have reported that various extracts Ficus exasperata exhibited better hypoglycemic and ameliorative effects than standard antidiabetic drugs and suggested further studies to isolate and characterize the components of the plants responsible for the activities. This study was therefore designed to isolate and conduct preliminary characterization of the components of F. exasperata that are responsible for the hypoglycemic and ameliorative properties on the complication of diabetes mellitus in albino rats.
Methanol extract of F. exasperata was partitioned using ethyl acetate and n hexane. Preliminary in vivo study to assess the hypoglycemic potential of the partitions was conducted using male albino rats as model. The ethyl acetate partition (with the best hypoglycemic effect) was further fractionated by column and thin layer chromatography. Fractions obtained were further used for in vivo study. Adult male rats were divided into 4 treatment and one control groups (n = 5 rats). The treatment groups were induced by a single intraperitoneal injection of 150mg/kg alloxan monohydrate. The effects of treatment with 300mg/kg of the fractions and 10mg/kg of a standard antidiabetic drug (glibenclamide) on the blood glucose, haematology, liver enzymes, lipid profile and histopathology of some organs were thereafter studied.
all treated rats responded positively to treatment with the fraction and hyperglycemia was reversed within 7 days of treatment. Treatment with the fraction induced significantly higher (p < 0.05) haemopoetic values and lower hyperlipidemia values than the standard antidiabetic drug. Various degrees of degeneration were observed in the pancreas, kidney, liver and heart of treated and untreated diabetic rats. However, the degrees of degenerations were milder in rats treated with the fraction compared with the rats treated with glibenclamide. The fraction contained 7 compounds and the most prominent compound is gamma-Sitosterol with a percentage of 25.49.
result therefore showed that the fraction is a good candidate drug for the treatment of diabetes mellitus.
The 3rd Africa Diabetes Congress, Yaounde, Cameroon (Yaounde)
Dates: 19 Apr 17 - 22 Apr 17
Venue: Yaounde Congress Hall
Organizers: Africa Diabetes Congress Limited
Contact person: Prof. Jean Claude Mbanya (email@example.com)