Proceedings of 1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases (Gabès, 2017)

Abstract

Comparative evaluation of diagnostic approaches for malaria

Cite this: Pan African Medical Journal - Conference Proceedings. Nov 2017; 4(4): 13. doi:10.11604/pamj.cp.2017.4.13.444

Submitted: 23 Oct 17   Accepted: 03 Nov 17   Published: 15 Nov 17

Key words: Plasmodium, malaria, diagnostic approaches

© Hagen Frickmann et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Available online at: http://www.proceedings.panafrican-med-journal.com/conferences/2017/4/13/abstract

Corresponding author: Hagen Frickmann, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (frickmann@bnitm.de)

This abstract is published as part of the proceedings of 1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases(TUNISIA, )

Comparative evaluation of diagnostic approaches for malaria

Hagen Frickmann1,&

 

1Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

 

 

&Corresponding author
Hagen Frickmann, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

 

 

Abstract

Introduction: microscopy is the backbone of traditional malaria diagnostics. However, availability of technical assistants on military deployments who are well experienced with malaria microscopy is scarce. Therefore, molecular diagnostic procedures are desirable which are widely independent from the skills of the investigator.

 

Methods: in-house and commercial PCRs systems and various nucleic acid extraction procedures were compared.

 

Results: there was no relevant difference in the performance of a well-established in-house PCR approach in comparison with a commercial PCR kit for the detection of malaria. Automated nucleic acid extraction from blood samples proved to be as reliable as manual column-based nucleic acid extraction.

 

Conclusion: the results demonstrate the basic suitability of molecular diagnostic approaches for the detection of malaria with a high degree of automation for the deployment setting. Potential future optimization may include modern approaches like loop-mediated amplification (LAMP), which is currently under investigation at our department.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases ()

Country: TUNISIA

Dates: 23 Oct 16 - 25 Oct 16

Venue:

Organizers:

Secretariat: dgsante_cmed@defense.tn

Contact person: Pr Salem Bouomrani (Salembouomrani@yahoo.fr)