Lower activation-induced T-cell apoptosis is related to the pathological immune response in secondary infection with hetero-serotype Dengue virus
Jintao Li1,&, Huacheng Yan1, Tiantian Yu1, Hongxia Guo1, Nan Ye1, Yue Chen1, Ruiwen Ren1
1Institute of Tropical Medicine, Third Military Medical University, China
Jintao Li, Institute of Tropical Medicine, Third Military Medical University, China
the available evidence suggests that dengue virus-specific T-lymphocytes
and cytokine storm play a pivotal role in the immune-pathogenesis of plasma
leakage. Investigations are underway to identify the immune profiles associated
with increased or decreased risk for severe disease.
in this study, CD14+ cells from the peripheral blood mononuclear cells (PBMCs) of patients who recovered from DENV-1 infection were infected with DENV-1 or DENV-2 and co-cultured with memory T-cells.
we found that secondary infection with DENV-2 suppresses the cell reproductive
capacity but forms more cell clones and more functional cells to produce
more pro-inflammatory factors (IFN-γ, TNF-α, IL-6, IL-8, IL-12 and
IL-17) and less regulatory cytokines (IL-10, TGF-β) which results in higher
viral replication compared to secondary infection with DENV-1. Memory dengue
T-cells which are induced in a primary dengue virus infection are reactivated
by the heterologous serotype of dengue virus and antigen presenting cells
(APCs) during a secondary infection. Dramatically, less apoptosis and more
continuous activation of T-cells in secondary infection with hetero-serotype
observed. This discovery which has not been reported previously may be the
and vital interpretation for the cytokine storm and severe symptoms observed
in secondary infection with DENV.
secondary infection with hetero-serotype DENV elicits the relatively pathological
immune response while secondary infection with homologous-serotype DENV induces
the relatively protective immune response by activation-induced cell death
(AICD) of T-cells.
1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases ()
Dates: 23 Oct 16 - 25 Oct 16
Contact person: Pr Salem Bouomrani (Salembouomrani@yahoo.fr)