Proceedings of 1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases (Gabès, 2017)

Abstract

Lower activation-induced T-cell apoptosis is related to the pathological immune response in secondary infection with hetero-serotype Dengue virus

Cite this: Pan African Medical Journal - Conference Proceedings. Nov 2017; 4(4): 44. doi:10.11604/pamj.cp.2017.4.44.416

Submitted: 20 Oct 17   Accepted: 03 Nov 17   Published: 21 Nov 17

Key words: Dengue, serotype DENV, immune response, apoptosis

© Jintao Li et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Available online at: http://www.proceedings.panafrican-med-journal.com/conferences/2017/4/44/abstract

Corresponding author: Jintao Li, Institute of Tropical Medicine, Third Military Medical University, China (jintaoli2006@sohu.com)

This abstract is published as part of the proceedings of 1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases(TUNISIA, )

Lower activation-induced T-cell apoptosis is related to the pathological immune response in secondary infection with hetero-serotype Dengue virus

Jintao Li1,&, Huacheng Yan1, Tiantian Yu1, Hongxia Guo1, Nan Ye1, Yue Chen1, Ruiwen Ren1

 

1Institute of Tropical Medicine, Third Military Medical University, China

 

 

&Corresponding author
Jintao Li, Institute of Tropical Medicine, Third Military Medical University, China

 

 

Abstract

Introduction: the available evidence suggests that dengue virus-specific T-lymphocytes and cytokine storm play a pivotal role in the immune-pathogenesis of plasma leakage. Investigations are underway to identify the immune profiles associated with increased or decreased risk for severe disease.

 

Methods: in this study, CD14+ cells from the peripheral blood mononuclear cells (PBMCs) of patients who recovered from DENV-1 infection were infected with DENV-1 or DENV-2 and co-cultured with memory T-cells.

 

Results: we found that secondary infection with DENV-2 suppresses the cell reproductive capacity but forms more cell clones and more functional cells to produce more pro-inflammatory factors (IFN-γ, TNF-α, IL-6, IL-8, IL-12 and IL-17) and less regulatory cytokines (IL-10, TGF-β) which results in higher viral replication compared to secondary infection with DENV-1. Memory dengue virus-specific T-cells which are induced in a primary dengue virus infection are reactivated by the heterologous serotype of dengue virus and antigen presenting cells (APCs) during a secondary infection. Dramatically, less apoptosis and more continuous activation of T-cells in secondary infection with hetero-serotype DENV were observed. This discovery which has not been reported previously may be the reasonable and vital interpretation for the cytokine storm and severe symptoms observed in secondary infection with DENV.

 

Conclusion: secondary infection with hetero-serotype DENV elicits the relatively pathological immune response while secondary infection with homologous-serotype DENV induces the relatively protective immune response by activation-induced cell death (AICD) of T-cells.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1st International Military Congress of Tropical Medicine and Sub-Saharan Diseases ()

Country: TUNISIA

Dates: 23 Oct 16 - 25 Oct 16

Venue:

Organizers:

Secretariat: dgsante_cmed@defense.tn

Contact person: Pr Salem Bouomrani (Salembouomrani@yahoo.fr)