Proceedings of Nigeria Centre for Disease Control/Nigeria Field Epidemiology and Laboratory Training Programme 2nd Annual Scientific Conference (Abuja, 2018)

Opening ceremony

Risk factors for methicillin-resistant staphylococcus aureus (MRSA) colonization in donkeys and human contacts in Ebonyi State, 2016

Cite this: Pan African Medical Journal - Conference Proceedings. Mar 2018; 8(8): 1. doi:10.11604/pamj.cp.2018.8.1.680

Submitted: 19 Jan 18   Accepted: 26 Jan 18   Published: 14 Mar 18

Key words: Methicillin-resistant <i>staphylococcus aureus</i>, donkey, multidrug- resistance

© Okoro Aja Nwenyi et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Available online at: http://www.proceedings.panafrican-med-journal.com/conferences/2018/8/1/abstract

Corresponding author: Okoro Aja Nwenyi, Veterinary Services Department, Ministry of Agriculture, Abakaliki, Ebonyi State, Nigeria (okoronwenyi@gmail.com)

This abstract is published as part of the proceedings of Nigeria Centre for Disease Control/Nigeria Field Epidemiology and Laboratory Training Programme 2nd Annual Scientific Conference(NIGERIA, )

Risk factors for methicillin-resistant staphylococcus aureus (MRSA) colonization in donkeys and human contacts in Ebonyi State, 2016

Okoro Aja Nwenyi1,&, Gabriel Ogundipe2, Ikeoluwapo Ajayi3, Akanni Abdulazeez4

 

1Veterinary Services Department, Ministry of Agriculture, Abakaliki, Nigeria, 2Veterinary Teaching Hospital, University of Ibadan, Nigeria, 3Department of Epidemiology and Medical Statistics, University of Ibadan, Nigeria, 4Department of Surgery, Federal Teaching Hospital, Abakaliki, Nigeria

 

 

&Corresponding author
Okoro Aja Nwenyi, Veterinary Services Department, Ministry of Agriculture, Abakaliki, Ebonyi State, Nigeria

 

 

Abstract

Introduction: methicillin-resistant Staphylococcus aureus (MRSA) emerged as a hospital problem in the 1960s and has spread from hospitals to the community and to livestock. Livestock-associated MRSA poses an occupational risk to abattoir workers. This study was carried out to determine the prevalence of MRSA colonizing donkeys in Ebonyi State; the prevalence of MRSA in the humans in contact and the risk factors for colonization in the human subjects.

 

Methods: a cross sectional study was conducted in a slaughter slab in Ohaukwu Local Government Area using cluster sampling technique. 195 donkeys and 50 slab workers were selected. Nasal swabs were obtained and cultured to identify Staphylococcal organisms. Antibiotic sensitivity tests using Kirby-Bauer disc diffusion method was carried out on the MRSA positive isolates to determine their resistance profiles. Interviewer administered questionnaires was used to obtain data on risk factors from the human subjects. Data was analyzed using means, standard deviation and proportions as well as chi square test. The criterion for statistical significance was p < 0.5.

 

Results: four breeds were identified (Idabari, Fari, Duni and Auraki). 144 (73.9%) jennies (female donkeys) and 51 (26.2%) jacks (male donkeys) were sampled. Prevalence of MRSA in donkeys was 79.5%. Breed and sex were not significantly associated with MRSA colonization in donkeys (X2 = 0.150, P = 0.698, X2= 1.136, P = 0.286). Mean age of slaughter slab workers was 32.4 9.7, 72% females and 28% males. Majority (84%) were married. Mean number of years of work at slaughter slab was 7.6 6.6. Majority (82%) did not use any personal protective equipment while at work. The prevalence of MRSA slaughter slab workers was 54%. Donkey MRSA isolates showed multidrug- resistance (100% to ampicillin, bacitracin, oxacillin and cefoxitin; 99% cloxacillin, and mupirocin; 96% vancomycin). Susceptibility to gentamicin was 96%.

 

Conclusion: the study identified high prevalence of MRSA colonization in donkeys and human contacts and high resistance of donkey MRSA to antimicrobials that are not routinely used in donkeys. Molecular characterization of MRSA at donkey-human interface is recommended to understand the direction of spread of MRSA at this interface.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Nigeria CDC/Nigeria Field Epidemiology and Laboratory Training Programme 2<sup>nd</sup> Annual Scientific Conference (Abuja)

To create a platform for epidemiologists and public health physicians to share their scientific works, NCDC/NFELTP organized the 2nd annual scientific conference with the theme "strengthening one health through field epidemiology training" at Transcorp Hilton Hotel, Abuja, Nigeria from July 5-7, 2017. The objectives of the conference were to provide residents and graduates a forum to share findings from their field activities; provide training opportunity for trainees on scientific communication; provide an opportunity for epidemiological networking as well as create a forum to discuss pertinent public health issues. In attendance were dignitaries from the United States Centers for Disease Control and Prevention (CDC), USAID, WHO, Africa CDC, Ministries Departments and Agencies, University officials and other implementing partners. With the current rise in zoonotic diseases, the conference also featured a two-day pre-conference workshop on One Health which prioritized zoonotic infectious diseases in Nigeria using standardized prioritization methods. A second workshop focused on antimicrobial resistance. There were 38 oral presentations, 60 poster presentations and 2 plenary sessions. The presentations covered various sub-themes ranging from outbreak investigations, case management, health system strengthening, vaccine preventable diseases, communicable diseases and surveillance. The conference featured a National Night and climaxed with awards to outstanding presenters.

Country: NIGERIA

Dates: 05 Jul 17 - 07 Jul 17

Venue: Transcorp Hilton Hotel

Organizers: Nigeria Centre for Disease Control / Nigeria Field Epidemiology and Laboratory Training Programme

Secretariat: gchinyere@afenet.net

Contact person: Dr Patrick Nguku (pnguku@afenet.net)