Conference abstract

Metabolic effects of add-on probiotics supplementation in Cameroonian patients with uncontrolled type 2 diabetes mellitus (T2DM)

Pan African Medical Journal - Conference Proceedings. 2017:2(15).31 Dec 2017.
doi: 10.11604/pamj-cp.2017.2.15.45

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Keywords: Probiotics, metabolic profile, hyperinsulinaemic-euglycaemic clamp, Type 2 Diabetes mellitus, Cameroon
Oral presentation

Untitled Document

Metabolic effects of add-on probiotics supplementation in Cameroonian patients with uncontrolled type 2 diabetes mellitus (T2DM)

Raicha Namba1,&, Eugène Sobngwi2, Andréa Omengue3, Armand Mbanya4, Jean-Claude Njabou Katte5, Jean Claude Mbanya6

1Department of Internal Medicine, Faculty of Medicine and Biomedical sciences, University of Yaoundé 1, Yaoundé, Cameroon, 2National Obesity Center, Yaoundé Central Hospital and Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1,Yaoundé, Cameroon, 3Department of Internal Medicine, Faculty of Medicine and Biomedical sciences, University of Yaoundé I, Yaoundé, Cameroon, 4Albert Einstein College of Medicine, New York, United States of America, 5Department of Public Health, Faculty of Medicine and Biomedical sciences, University of Yaoundé I, Cameroon, 6National Obesity Center, Yaoundé Central Hospital and Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon

&Corresponding author
Raicha Namba, Department of Internal Medicine, Faculty of Medicine and Biomedical sciences, University of Yaoundé 1, Yaoundé, Cameroon

Abstract

Introduction: the aetiology and progression of diabetes mellitus is related to the alteration of gut microbiota. It is therefore expected that, restoring gut microbiota will improve metabolic profile in type 2 diabetes mellitus (T2DM) patients. The main objective of the study was to evaluate the effects of add-on probiotics supplementation containing Lactobacillus spp. and Bifidobacterium spp. on metabolic profile in uncontrolled T2DM patients.

Methods: we undertook a single arm non-randomized clinical trial. Ten T2DM patients received the allocated treatment. The intervention was a daily intake of probiotics supplementation over an eight-week period. We assessed before and after intervention: insulin sensitivity using the hyperinsulinaemiceuglycaemic clamp, insulin secretion using a standardized mixed meal tolerance test, glycated haemoglobin, serum lipid profile and body composition. In addition, clinical tolerance, ALAT level and serum creatinine were assessed at week 0, 4, 8, 10 and 14 for safety purpose.

Results: results revealed that insulin sensitivity showed a trend towards increase by 18% after intervention, from; 9.1(12.6-8.3) mg/kg/min to 11.3(15.8-9.7) mg/kg/min, p = 0.07. Insulin secretion increased significantly after intervention, t90: 0.54 [0.93-0.41]µg/L versus 1.59 [2.58-0.53]µg/L, p = 0.021 and t120: 0.49 (0.71-0.28)µg/L versus 1.49 (2.28-0.51)µg/L, p = 0.038. Plasma glucose reduced significantly after intervention; from 6.9 (8.1-4.9)mmol/L before to 5.8 [6.3-4.3]mmol/L after intervention, p < 0.05 at t0 and from 7.6 [9.0-5.9]mmol/L before to 6.2 (7.4-5.3)mmol/L after intervention, p = 0.04 at t30. There was a significant reduction of 25units in HbA1c, from 80.9 (86.0-73.5)mmol/mol to 56.3 (67.2-45.1)mmol/mol, p=0.005. Total cholesterol and LDL cholesterol were also significantly lower, from 4.2(4.4-3.8)mmol/L to 3.5[3.9-3.3]mmol/L, p = 0.015 and from 2.3 (2.6-2.0)mmol/L to 1.8(2.2-1.6)mmol/L, p = 0.015 respectively.

Conclusion: the use of probiotics induced a significant improvement in glucose metabolism and insulin secretion with a major HbA1c reduction over an eight-week intervention.