Conference abstract

Immune suppression in severe infection: level of immunosuppressive cytokine IL10 and IL4 during sepsis and correlation with sequential organ failure assessment score

Pan African Medical Journal - Conference Proceedings. 2017:4(49).27 Dec 2017.
doi: 10.11604/pamj-cp.2017.4.49.420

Contact the corresponding author
Keywords: Severe infection, spesis, immune response, IL10, SOFA
Abstract

Immune suppression in severe infection: level of immunosuppressive cytokine IL10 and IL4 during sepsis and correlation with sequential organ failure assessment score

Mouna Ben Azaiz1,2,3,&, Iheb Labbene3,4, Zied Hajjej3,4, Oueslati Riadh2, Ezzeddine Ghazouani1, Moustapha Ferjani3,4

1Department of immunology, Military Hospital of Tunis, Tunis, Tunisia, 2Department of Immunology, Faculty of Science of Bizerte, Bizerte, Tunisia, 3Research Laboratory LR12DN01, Military Hospital of Tunis, Tunis, Tunisia, 4Department of Anesthesiology and Critical Care Medicine, Military Hospital of Tunis, Tunis, Tunisia

&Corresponding author
Mouna Ben Azaiz, Department of immunology, Military Hospital of Tunis, Department of Immunology, Faculty of Science of Bizerte, Research Laboratory LR12DN01, Military Hospital of Tunis, Tunis, Tunisia

Abstract

Introduction: infectious diseases remain a major public health issue in developed and developing countries. The original conceptualization of sepsis as infection with at least 2 of the 4 SIRS criteria focused solely on inflammatory excess. However, the validity of SIRS as a descriptor of sepsis pathobiology has been challenged. Sepsis is now recognized to involve early activation of both pro- and anti-inflammatory responses. Few studies assessed immunosuppressive cytokines during sepsis and their correlation with sequential organ failure assessment score (SOFA). The aim of our work was to study these cytokines mainly IL10 and Il4 during sepsis and correlation with SOFA score.

Methods: all patients in sepsis during a period of 12 months were enrolled in a prospective clinical study. Blood samples were collected at two times for IL10 and IL4 (H0 and H24). H0 is the first time for the diagnosis of sepsis in the patients. Serum level was measured by enzyme immunoassay method (EIA) using a commercial Kit (Biosystem ģ, Barcelona, Spain). SOFA score was calculated for each patient at H0, H24 and H48. We had used SPSS 11.0. Student T. test was statistically significant if p < 0. 05. Correlations between variables were determined by using the Pearson correlation analysis for normally distributed variables.

Results: we have included thirty-one patients. There was a correlation between the level of IL10 and SOFA score at H0 (p = 0.039). There was an elevation of IL4 during the first 24h. The mean value of IL4 at H0 was higher than H24: respectively 14.99 pg/ml and 17.66 pg/ml but deference is not significant (p = 0.66). There isnít a correlation between IL4 and SOFA score at different time.

Conclusion: there was a correlation between the level of IL10 and SOFA score at H0. We found also an elevation of IL4 during first 24h but we donít found a correlation with SOFA score. Although these are preliminary results, these findings can contribute to a better understanding of physiopathology and outcome. This will allow us to find a new therapeutic target for Immunomodulation, which is the next step in sepsis treatment.