Conference abstract

Genetic risk for Ketosis-Prone Diabetes (KPD) in a Cameroonian population: role of rs4731702(C/T) polymorphism of Krüppel-Like Factor 14 (KLF14) gene, master trans regulator

Pan African Medical Journal - Conference Proceedings. 2021:11(27).30 Dec 2021.
doi: 10.11604/pamj-cp.2021.11.27.953

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Keywords: Ketosis prone diabetes, genetic, KLF14, Cameroon
Poster

Genetic risk for Ketosis-Prone Diabetes (KPD) in a Cameroonian population: role of rs4731702(C/T) polymorphism of Krüppel-Like Factor 14 (KLF14) gene, master trans regulator

Magellan Guewo-Fokeng1,2, Wilfred Fon Mbacham1,2, Barbara Atogho Tiedeu1,2, Eric Lontchi-Yimagou1,2, Jean Claude Mbanya2,3,4, Eugene Sobngwi2,3,4,&

1Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon, 2The Biotechnology Center, University of Yaoundé I, Nkolbisson, Yaoundé, Cameroon, 3Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon, 4National Obesity Center, Diabetes, Endocrinology and Metabolic Diseases, Yaoundé Central Hospital, Yaoundé, Cameroon

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Introduction: the number of genetic risk variants identified in this region compared to others in the world is very insignificant, with only two (rs7903146 and rs12255372 on TCF7L2 gene) in Cameroon in Type 2 Diabetes Mellitus susceptibility. This study aimed to assess the association between rs4731702(C/T) polymorphism of the Krüppel Like Factor 14 (KLF14) gene with Ketosis Prone Diabetes (KPD) in a Cameroonian population. This will help to create a large genomic research database, improve our understanding of the genetic risk factors for the disease and make a contribution to the plan to personalized medicine. Methods: this case-control study included 34 KPD patients and 71 healthy normoglycemic controls who are all unrelated Cameroonian adults (aged ≥ 24 years). Demographic, clinical and biological data were collected to determine phenotypic traits. Biochemical analyses were performed using a spectrophotometer with Chronolab kits. KLF14 rs4731702(CT) genotypes were determined using Polymerase Chain Reaction follow by Restriction Fragment Length Polymorphism (PCR-RFLP). Results: of the KLF14 rs4731702(C/T) polymorphism, the T allele was associated with KPD, whereas (70.59% KPD patients vs. 30.99% healthy controls, OR=5.345 and p<0.0001) the C allele was protective (29.41% KPD patients vs. 69.01% healthy controls, OR=0.187 and p<0.0001). The susceptibility to KPD was higher among subjects who had the TT genotype with OR=4.756 (95%CI [1.984-11.40], p=0.0005). Conclusion: this study showed, for the first time, the association between KLF14 rs4731702(C/T) gene polymorphism with KPD in a Cameroonian population. It is a promising target for personalized medicine through the development of clinical genetic testing.