Conference abstract

The Krüppel-Like Factor 14, rs4731702(C/T) gene polymorphism and its implication in the predisposition of Cameroonian population to type 2 diabetes mellitus: a case control study

Pan African Medical Journal - Conference Proceedings. 2021:11(28).30 Dec 2021.
doi: 10.11604/pamj-cp.2021.11.28.954

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Keywords: Type 2 diabetes mellitus, genetic , KLF14, Cameroon
Poster

The Krüppel-Like Factor 14, rs4731702(C/T) gene polymorphism and its implication in the predisposition of Cameroonian population to type 2 diabetes mellitus: a case control study

Magellan Guewo-Fokeng1,2, Eugene Sobngwi2,3,4, Barbara Atogho Tiedeu1,2, Jean Claude Mbanya2,3,4, Wilfred Fon Mbacham1,2,&

1Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon, 2The Biotechnology Center, University of Yaoundé I, Nkolbisson, Yaoundé, Cameroon, 3Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon, 4National Obesity Center, Diabetes, Endocrinology and Metabolic Diseases, Yaoundé Central Hospital, Yaoundé, Cameroon

&Corresponding author

Introduction: the aim of the present study was to investigate the relationship between rs4731702(C/T) polymorphism of the Krüppel Like Factor 14 (KLF14) gene with type 2 diabetes mellitus (T2DM) in a Cameroonian population. Methods: this case-control study included 85 T2DM patients and 95 healthy normoglycemic controls. They were all unrelated, of Cameroonian origin, and adults aged 24 years old and above. Demographic, clinical and biological data were collected, and biochemical explorations were performed using enzymatic colorimetric methods. The genotyping of KLF14 rs4731702(CT) gene polymorphism was done by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Results: in comparing the Cameroonian population that consisted of 85 patients with T2DM and 95 healthy controls, the minor or risk allele of the rs4731702(C/T) polymorphism of the KLF14 gene was T (63.53% diabetic patients vs. 26.32% healthy controls, OR=4.877 and p<0.0001) while the protective allele was C (36.47% diabetic patients vs. 73.68% healthy controls, OR=0.205 and p<0.0001). The susceptibility to T2DM was higher among subjects having the CT and TT genotypes with OR=2.721 and p=0.0145) and OR=3.907 and p<0.0001) respectively. This gene polymorphism was not preferentially associated with a specific diabetes phenotype. Conclusion: in conclusion, this study showed for the first time the association between KLF14 rs4731702(C/T) gene polymorphism and T2DM in the Cameroonian population. This gene polymorphism could be a promising target for personalized medicine through the development of clinical genetic testing.