Conference abstract

Genotypic combination from TCF7L2-rs7903146(C/T), KLF14-rs4731702(C/T) and PPAR-γ2-rs1801282(C/G) gene polymorphisms in the predisposition to type 2 diabetes in a Cameroonian population

Pan African Medical Journal - Conference Proceedings. 2021:11(29).30 Dec 2021.
doi: 10.11604/pamj-cp.2021.11.29.955

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Keywords: Type 2 diabetes mellitus, Genetic association, KLF14, TCF7L2, PPAR-γ2, Sub-Saharan Africa, Cameroon
Poster

Genotypic combination from TCF7L2-rs7903146(C/T), KLF14-rs4731702(C/T) and PPAR-γ2-rs1801282(C/G) gene polymorphisms in the predisposition to type 2 diabetes in a Cameroonian population

Magellan Guewo-Fokeng1,2, Eugene Sobngwi2,3,4, Barbara Atogho Tiedeu1,2, Wilfred Fon Mbacham1,2, Jean Claude Mbanya2,3,4,&

1Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon, 2The Biotechnology Center, University of Yaoundé I, Nkolbisson, Yaoundé, Cameroon, 3Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon, 4National Obesity Center, Diabetes, Endocrinology and Metabolic Diseases, Yaoundé Central Hospital, Yaoundé, Cameroon

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Introduction: the aim of this study was to investigate the implication of the combination of TCF7L2-rs7903146(C/T), KLF14-rs4731702(C/T) and PPAR- Genotypic combination from TCF7L2-rs7903146(C/T), KLF14-rs4731702(C/T) and PPAR-γ2-rs1801282(C/G) gene polymorphisms in the predisposition to type 2 diabetes in a Cameroonian population 2-rs1801282(C/G) gene polymorphisms in the predisposition to T2D in a Cameroonian population. Methods: this case-control study included 198 type 2 diabetic patients and 182 non-diabetic volunteers as controls were recruited for this study. They were Cameroonian, all 18 years old and above. Demographic, clinical and biological data were collected, and biochemical explorations were performed using enzymatic colorimetric methods. Genotyping was done by PCR-RFLP. Results: the metabolic profile of the patients with T2D was in poorer shape than that of the control group. The KLF14 rs4731702(C/T) gene polymorphism was strongly associated with T2D (OR=5.857; p<0.0001) in this Cameroonian population, and not dependent on any specific T2D phenotype. The combined effect of the rs4731702(C/T) of the KLF14 gene, the rs7903146(C/T) of the TCF7L2 gene and the rs1801282(C/G) of the PPAR-γ2 gene showed that individuals with the TTrs4731702 and TTrs7903146 (OR=5.167 and p=0.0125) genotypic combination, like individuals with the TTrs4731702 and CCrs1801282 combination (OR=9.203 and p=0.0003), and more interestingly those presenting the TTrs4731702_CTrs7903146_CCrs1801282 combination (100%), stand a greater risk of developing T2D. Conclusion: The KLF14 rs4731702(C/T) gene polymorphism and certain genotypic combinations of KLF14 rs4731702(C/T), TCF7L2 rs7903146(C/T) and PPAR-γ2 rs1801282(C/G) gene polymorphisms may predispose this population to T2D.