Conference abstract

KLF14, TCF7L2, PPARG, HSP70 genes/proteins and beneficial effect of heat therapy for Type 2 diabetes mellitus: protein-protein interactions, plausible explanation

Pan African Medical Journal - Conference Proceedings. 2021:11(30).30 Dec 2021.
doi: 10.11604/pamj-cp.2021.11.30.956

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Keywords: Heat therapy, type 2 diabetes mellitus, protein-protein interactions
Poster

KLF14, TCF7L2, PPARG, HSP70 genes/proteins and beneficial effect of heat therapy for Type 2 diabetes mellitus: protein-protein interactions, plausible explanation

Magellan Guewo-Fokeng1,2, Eugene Sobngwi2,3,4, Barbara Atogho Tiedeu1,2, Jean Claude Mbanya2,3,4, Wilfred Fon Mbacham1,2,&

1Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon, 2The Biotechnology Center, University of Yaoundé I, Nkolbisson, Yaoundé, Cameroon, 3Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon, 4National Obesity Center, Diabetes, Endocrinology and Metabolic Diseases, Yaoundé Central Hospital, Yaoundé, Cameroon

&Corresponding author

Introduction: the aim of the present study is to provide a contribution for understanding the pathophysiology of T2DM and determine the implication of HSP70 in the beneficial effect of heat therapy for T2DM. Methods: Data were generate by the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) software version 10.0 was used to identify the known and predicted protein-protein interaction in the KLF14, TCF7L2, PPARG and HSP70 proteins network, Under the active prediction methods (Gene Fusion, Neighborhood, Co-occurrence, Co-expression, Experiments, Databases and Text Mining) as interaction sources, Medium Confidence (0.400) and max number of interactions to show no more than 50 at the first shell and none at the second shell parameters. Results: fifty (50) proteins were identified to interact with these four (04) proteins (KLF14, TCF7L2, PPARG and HSP70) resulting a network diagram with 54 nodes (gene/proteins) including the KLF14, TCF7L2, PPARG and HSP70 nodes and 485 edges (representing protein-protein associations) or interactions. The network shows that HSP70 is strongly interact with another heat shock protein called HSP90, also with HSPBP1, cytoplasmic co-chaperone 1, Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Conclusion: base on the data generated by the present study, it is evident that the benefit effect obtain by heat therapy on T2DM may be due to the protein-protein interactions establish between certain proteins implicated in the cell live, in the susceptibility of T2DM and Heat Shock Proteins with a particularity of HSP70.