Conference abstract

Detection of anti-Sars-Cov-2 antibodies in hospital employees of a public health facility in Douala (Cameroon)

Pan African Medical Journal - Conference Proceedings. 2023:16(5).15 Mar 2023.
doi: 10.11604/pamj-cp.2023.16.5.1860
Archived on: 15 Mar 2023
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Keywords: Anti-SARS-COV-2 antibodies, hospital staff, Douala
Oral presentation

Detection of anti-Sars-Cov-2 antibodies in hospital employees of a public health facility in Douala (Cameroon)

Jean Pierre Nda Mefo’o1,2, Vanessa Kengne Ngueko2,&, Elodie Ngo Malabo1, Emmanuel Roddy Mengue1, Catherine Akono Ndi1, Henry Namme Luma3, Cécile Okalla Ebongue1,2

1Laboratoire de Biologie Clinique, Hôpital Général de Douala, Douala, Cameroun, 2Département des Sciences Biologiques, Faculté de Médecine et des Sciences Pharmaceutiques, Université de Douala, Douala, Cameroun, 3Service de Médecine Interne, Hôpital Général de Douala, Douala, Cameroun

&Corresponding author

Introduction: COVID-19 has affected excessively medical personnel and this infection has induced an immune response in most individuals by producing antibodies against SARS-CoV-2.

Methods: we conducted a cross-sectional study over a period from December 2021 to June 2022 at the Douala General Hospital. Staff samples were taken in the various departments. Sociodemographic data were collected by interviewing participants. The biological samples obtained by venipuncture of 5 ml of blood in a tube of lithium heparin and were analyzed at the clinical biology laboratory of the same Hospital. Quantitative IgG and qualitative IgM assays were performed using the Enzyme Linked Fluorescent Assay (ELFA) method on an automaton (bio Mérieux VIDAS™ immunoassay). The data obtained were analyzed with SSPS 20 software.

Results: a total of 96 participants were included in the study. The female sex was the most represented (sex ratio: 0.65). The average age was 37.7 ± 9.3 years with a predominance of the age group 32 to 42 years (46.9%). Most of the staff came from Medical Services (38.5%) and nurses were the most represented (38%). The overall prevalence was 90.6% for IgG and 3.13% for IgM. IgG titration showed positive levels ranging from 22 to 1157 BAU/ml with the majority of cases between 301-600 BAU/ml (39.6%). Prior vaccinated people had IgG antibodies in 94.0% of cases. For staff who had COVID-19 within 4 to 9 months and more than 12 months, 100% had positive IgG levels. The highest levels of IgG were observed in doctors (100%) and in medical services (97.3%). IgM was detected in 3 (3.13%) personnel (2 vaccinated and 1 infected with COVID-19 four months ago).

Conclusion: most of the personnel tested were carriers of IgG antibodies. The IgG level was not significantly associated with age, gender, professional category, or assignment services in the hospital.